A portion of the citrate synthase gene of Rickettsia amplified by the
polymerase chain reaction (PCR). Demonstrates application of buffer penetrating hard cuticle of chiton to preserve nucleic acids in intracellular parasites stored at room temperature for 5 years.
Source: Lane 1, molecular ladder. Lane 2, positive control. Lane 3, negative
control. Lanes 4 - 16, positive samples from rickettsial infected tick vectors.